P4

Calcium and anatomical imaging of the live mouse retina during experimental autoimmune encephalomyelitis: insights into the cascade of neurodegeneration

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The figure above shows an in vivo two-photon microscopic imaging of retinal blood vessels, retinal ganglion cells and microglia.

Project 4 (Winkler/Williams; Neurology Heidelberg) applies multiphoton laser scanning microscopy as a newly developed technique for high 3D-resolution retinal imaging of live mice with autoimmune optic neuritis/experimental autoimmune encephalomyelitis.
Through the selective loading of retinal ganglion cells with intracellular calcium indicators, and novel genetically encoded calcium indicators, the sequence of events of intercellular calcium waves, microglial activation, T cell interactions and blood brain barrier (BBB) disruption can be monitored in real time and with high anatomical precision and their impact on neurodegeneration determined.