P6

Calcium-dependent T-cell activation in multiple sclerosis

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The figure above show an analysis by flow cytometry of cells producing cytokines IFN-g, or IL-17 or expressing the transcription factor FoxP3 (left panel) and of store operated Ca2+ entry (right panel) from human CD4 stimulated and cultured under conditions for polarizing into Treg (red), Th1 (grey),or Th17 (green) compared to the non-polarized cells (black).

Project 6 analyses calcium signalling signatures, store-operated calcium entry mechanisms and their redox sensitivity as well as cleavage targets of caspases in healthy human and rodent Th1, Th2, Th17 and Treg cells compared to those obtained during autoimmune optic neuritis and MS.

These findings will be correlated with T cell pathogenicity, T cell subtype fate determination, the ability of T cells to cross the blood brain barrier (BBB), and the extent of neurodegeneration.